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Welcome to the home of the Moorea Biocode LIMS (Laboratory Information Management) Software. The Biocode software comprises everything you need to manage your lab and sequence analysis workflows. This wiki serves both as a repository for the plugins themselves (with release notes and links to downloads), and an extensive online manual. You can download the plugin by following the Getting Started instructions in the green section on the right. If you run into trouble, or want to unlock the full power of the LIMS, read through the blue manual section on the right hand side. See www.mooreabiocode.org for details of the Moorea Biocode Project.


Getting Started

You will need to download three files, in addition to Geneious in order to use the Biocode plugin.


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  • The Biocode plugin itself
  • The Biocode Genbank Submission plugin, which allows you to submit your completed contigs to Genbank.
  • The MySQL Connector, which allows you to connect to FIMS and LIMS databases.

Download.png

You can download Geneious from http://www.geneious.com.

See Getting Started for detailed instructions on how to install and configure the plugins in Geneious.

Getting Help

The Biocode plugins are complex, and while learning how to use them can seem like a daunting task, help is available. Your first port of call should be the introduction video, which walks you through the plugins. You can find a more detailed reference in the manual (available on the right of this page), and a list of frequently asked questions below.

A user community, and technical support is available from http://connect.barcodeoflife.org/group/lims

Here, you can engage with the wider community, get help from experienced users, and make suggestions about how to improve the plugins.

If you have and questions or suggestions that you don't want to post to the community, you can email support@mooreabiocode.org

F.A.Q.

Frequently asked questions will appear here shortly.

Updates

Biocode Plugin 2.8.1 - 23 September 2014

  • Made 'Forward Plate' and 'Reverse Plate' fields on assembled sequences support more than one value.
  • Fixed a bug where workflows were being added to search results when downloading sequences
  • Made the 'Mark as Passed' operation work even when links to the original chromatogram documents has been lost.
  • Fixed a problem where a background maintenance task would fail when using the Built-in MySQL LIMS.
  • Other minor fixes and improvements.

Biocode Plugin 2.8.0 - 19 August 2014

  • Added '# Passed' and '# Sequences' fields to cycle sequencing plate views.
  • Added 'Forward Plate' and 'Reverse Plate' fields to assembled sequences.
  • Annotate from FIMS/LIMS now works with any FIMS field in addition to well number.
  • Added extraction barcode as a displayable field on all plate types.
  • Improved performance when using the Biocode FIMS.
  • Fixed a bug where the # Traces field would not be populated in the Tabular Plate View.
  • Fixed a bug where searching the Moorea FIMS on Collection Date could return additional results.
  • Other minor fixes and improvements.

See the Release Notes for details on previous releases

LIMS

Key Concepts - Key concepts that you need to know before using the biocode plugin.

Workflows - It is important that you understand how workflows work before entering data.

Entering Data - How to enter data into the LIMS, from your first plate through to cherry picking failed reactions for a second try.

Searching - How to intelligently search your data in the LIMS

Collating Workflows - Workflows can be combined into summary views for project managers and troubleshooters.

Printing - All views of the LIMS data are fully printable. This section explains how to get the most out of printing.

Export - The biocode plugin supports exporting plates and summary views to excel, as well as a selection of other formats.

Assembly

Assembly Workflow - A flow diagram describing the full assembly workflow.

Importing Trace Data - How to get your trace data into Geneious.

Binning - Group your traces/contigs based on overall quality to speed up the assembly workflow, and quickly identify problem sequences.

Trimming - Trim low quality data and primers from the ends of your sequences.

Mark as Pass/Fail - Mark sequences as having passed or failed in the LIMS

Assembly - Assembling the prepared reads into contigs.

Analysis

Viewing And Editing Contigs - How to make base calls, edit gaps etc.

Alignment Of Consensus Sequences - Directly compare your contigs by aligning their consensus sequences.

Verify Taxonomy And Loci - Use BLAST to check the taxonomy of your sequences.

Reporting

Collating Workflows - Workflows can be combined into summary views for project managers and troubleshooters.

Generating Reports Charts - Tabular reports and charts can be generated from field and lab data.

Submission

Preparing your documents for submission - Make sure your sequences are correctly annotated.

Genbank Submission - Submit your sequences.

Mark as Submitted - Mark your sequences as being submitted in the LIMS.